■ 基本信息
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細胞，信號通路報告載體 |
質(zhì)粒大小: | 4242bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 信號報告 |
片段類型: | Promoter |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | Fluc
|
■ 質(zhì)粒簡介
The pGL4.10[luc2] Vectorencodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.10[luc2] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK473pGL4.10[luc2]哺乳報告質(zhì)粒.txt
