■ 基本信息
啟動(dòng)子: | T7 |
復(fù)制子: | M13 |
質(zhì)粒分類(lèi): | 大腸桿菌載體；蛋白表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 7477bp |
質(zhì)粒標(biāo)簽: | Intein |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | 大腸桿菌 |
5'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì) |
備注: | 表達(dá)質(zhì)粒 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pTYB1 is an E. coli vector created for fusion protein expression and purification. A target gene can be inserted into the the ORF by use of the MCS upstream of the Sce VMA intein. The NdeI site in the MCS has the initiator sequence ATG that enables translation of the ORF. The chitin-binding domain(CBD) downstream of the intein results in a target protein-intein with C-terminal chitin beads that facilitate protein purification. Release of the protein is induced by the thiol cleavage activity of the intein.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK335pTYB1大腸表達(dá)質(zhì)粒.txt
