■ 基本信息
別名: | pGEX6P3;pGEX6P-3 |
啟動子: | Tac |
復制子: | pBR322 |
質粒分類: | 大腸桿菌載體；PGEX系列表達質粒 |
質粒大小: | 4983bp |
質粒標簽: | N-GST，N-PP/3C |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達宿主: | 大腸桿菌 |
誘導方式: | IPTG或乳糖及其類似物 |
5'測序引物: | pGEX5:GGGCTGGCAAGCCACGTTTGGTG |
3'測序引物: | pGEX3:CCGGGAGCTGCATGTGTCAGAGG |
備注: | 可用GST親和柱純化重組蛋白 |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
There are a range of Gluthatione Sepharose prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 μl use GST SpinTrap? microspin columns or GST MultiTrap? 4B 96-well plates. For sample volumes between 600 μl and 10 ml use GST GraviTrap? gravity flow column. Where sample volumes are above 10 ml, use LabMate? reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap 1 and 5 ml columns and GST HiPrep? FF 16/10 column are available for purification in a chromatography system such as the ?KTA? design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty columns with required buffers.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK139pGEX-6P-3大腸表達質粒.txt
