■ 基本信息
啟動(dòng)子: | T7 |
復(fù)制子: | pBR322 |
質(zhì)粒分類: | 大腸桿菌載體；克隆質(zhì)粒 |
質(zhì)粒大小: | 2464bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
5'測(cè)序引物: | Sp6:ATTTAGGTGACACTATAGAA |
3'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
備注: | 豐富的酶切位點(diǎn) |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 基因模板 |
片段類型: | cDNA
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pSP73 Vector offers a wide range of restriction sites, providing greater versatility in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA polymerase promoters and a unique multiple cloning region, which includes restriction sites for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning region.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列可參考（具體以測(cè)序?yàn)闇?zhǔn)）下載：
ZK123pSP73體外轉(zhuǎn)錄質(zhì)粒.txt
